MMBIO 221 FINAL EXAM STUDY GUIDE
2024
Who is the father of microscopy?
Antonie van Leeuwenhoek
Lamp:
produces visible light to illuminate the specimen
Condenser:
collects light and directs it through the stage opening (converges or focuses the rays of
light to a single point)
Iris diaphragm:
regulates the amount of light available to the objective lens
Factors that affect microscope function:
Resolving power, numerical aperture, magnification, working distance
Resolving power:
ability of a microscope to differentiate two closely spaced objects as being distinct from
one another, use of shorter wavelength of light increases resolving power, resolving
power also depends on a second factor: the numerical aperture
Resolving power _____ as magnification of the objective lens ____
increases, increases
Numerical aperture:
ability of a lens in a light microscope to gather light
Refraction:
light rays are bent or refracted as they travel through media like glass and air, amount of
bending is indicated by the refractive index. Immersion oil minimizes refraction
producing a clearer image with more detail.
Magnification:
product of the magnification of the ocular (10x) and objective lens
Working distance:
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